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mouse mammary carcinoma cell line 4t1  (ATCC)


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    ATCC mouse mammary carcinoma cell line 4t1
    Mouse Mammary Carcinoma Cell Line 4t1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+mammary+carcinoma+cell+line/4T1/pmc13195117-354-1-16
    Average 99 stars, based on 6909 article reviews
    mouse mammary carcinoma cell line 4t1 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Alveolar Macrophages Drive Hepatocellular Carcinoma Lung Metastasis by Generating Leukotriene B 4 .
    Article Snippet: RESEARCH ARTICLE | MARCH 01 2018 Alveolar Macrophages Drive Hepatocellular Carcinoma Lung Metastasis by Generating Leukotriene B 4 Takuto Nosaka; ... et. al J Immunol (2018) 200 (5): 1839–1852. https://doi.org/10.4049/jimmunol.1700544 Related Content Clinicopathological and Prognostic Signi cance of CCR5/CCL5 axis in Hepatocellular Carcinoma J Immunol (May,2019) Intratumoral Neoadjuvant Immunotherapy Using IL-12 and Dendritic Cells Is an Effective Strategy To Control Recurrence of Murine Hepatocellular Carcinoma in Immunosuppressed Mice J Immunol (July,2010) IL-12 Gene Therapy Is an Effective Therapeutic Strategy for Hepatocellular Carcinoma in Immunosuppressed Mice J Immunol (December,2004) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/200/5/1839/1438694/ji1700544.pdf by guest on 13 January 2025 The Journal of Immunology

    Multiple Displacement Amplification:

    Article Title: Cyclin-Dependent Kinase Inhibitors Function as Potential Immune Regulators via Inducing Pyroptosis in Triple Negative Breast Cancer
    Article Snippet: .. Mouse mammary carcinoma cell line (4T1), and human TNBC cell lines (BT549, MDA-MB-231) were purchased from ATCC. .. MDA-MB-231 were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum, 1‰ penicillin/streptomycin.

    Cell Culture:

    Article Title: Bitter Bean (Parkia speciosa) Aqueous Extract: A Potential Natural Compound Agent Against Mammary Carcinoma in BALB/c Mice
    Article Snippet: Bitter beans (Parkia speciosa Hassk., Fabaceae) are widely used in Asia for both cooking and traditional medicine.. Mammary carcinoma, a global threat to animal health, drives research into alternative cancer treatments due to the harsh side effects of chemotherapy.. Plant bioactive compounds (alkaloids, carotenoids, flavonoids, phenolics, saponins, and more) possess therapeutic effects, for instance, anti-carcinogenic, anti-mutagenic, anti-inflammatory, and antioxidant activities.

    Modification:

    Article Title: Bitter Bean (Parkia speciosa) Aqueous Extract: A Potential Natural Compound Agent Against Mammary Carcinoma in BALB/c Mice
    Article Snippet: Bitter beans (Parkia speciosa Hassk., Fabaceae) are widely used in Asia for both cooking and traditional medicine.. Mammary carcinoma, a global threat to animal health, drives research into alternative cancer treatments due to the harsh side effects of chemotherapy.. Plant bioactive compounds (alkaloids, carotenoids, flavonoids, phenolics, saponins, and more) possess therapeutic effects, for instance, anti-carcinogenic, anti-mutagenic, anti-inflammatory, and antioxidant activities.



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    Image Search Results


    Mouse 4T1 mammary carcinoma cells (a) and mouse HC11 mammary epithelial cells (b) were seeded into 96-well plates and treated with isorhamnetin (ISO) at indicated concentrations for 24, 48, and 72 h. Cell proliferation was assessed using the CCK-8 assay. Data are presented as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Isorhamnetin inhibits migration and promotes apoptosis via modulation of oxidative stress, mitochondrial dysfunction, and the TIGIT/CD155 axis in 4T1 breast cancer cells

    doi: 10.3389/fcell.2026.1829358

    Figure Lengend Snippet: Mouse 4T1 mammary carcinoma cells (a) and mouse HC11 mammary epithelial cells (b) were seeded into 96-well plates and treated with isorhamnetin (ISO) at indicated concentrations for 24, 48, and 72 h. Cell proliferation was assessed using the CCK-8 assay. Data are presented as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Article Snippet: The 4T1 mouse mammary carcinoma cell line and HC11 mouse thymic epithelial cell line were both purchased from KeyGEN BioTECH Co., Ltd., (Nanjing, China).

    Techniques: CCK-8 Assay, Control

    (a) Representative images of 4T1 cell migration after treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 12, 24, and 48 h, assessed by wound healing assay. Untreated cells served as the control group. Scale bar = 300 μm. (b) Quantitative analysis of cell migration, presented as a percentage relative to the control group. Data are expressed as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Isorhamnetin inhibits migration and promotes apoptosis via modulation of oxidative stress, mitochondrial dysfunction, and the TIGIT/CD155 axis in 4T1 breast cancer cells

    doi: 10.3389/fcell.2026.1829358

    Figure Lengend Snippet: (a) Representative images of 4T1 cell migration after treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 12, 24, and 48 h, assessed by wound healing assay. Untreated cells served as the control group. Scale bar = 300 μm. (b) Quantitative analysis of cell migration, presented as a percentage relative to the control group. Data are expressed as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Article Snippet: The 4T1 mouse mammary carcinoma cell line and HC11 mouse thymic epithelial cell line were both purchased from KeyGEN BioTECH Co., Ltd., (Nanjing, China).

    Techniques: Migration, Wound Healing Assay, Control

    (a) Representative images of colony formation in 4T1 cells following treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 48 h, assessed by colony formation assay. (b) Quantitative analysis of colony formation, presented as a percentage relative to the control group. Data are expressed as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Isorhamnetin inhibits migration and promotes apoptosis via modulation of oxidative stress, mitochondrial dysfunction, and the TIGIT/CD155 axis in 4T1 breast cancer cells

    doi: 10.3389/fcell.2026.1829358

    Figure Lengend Snippet: (a) Representative images of colony formation in 4T1 cells following treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 48 h, assessed by colony formation assay. (b) Quantitative analysis of colony formation, presented as a percentage relative to the control group. Data are expressed as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Article Snippet: The 4T1 mouse mammary carcinoma cell line and HC11 mouse thymic epithelial cell line were both purchased from KeyGEN BioTECH Co., Ltd., (Nanjing, China).

    Techniques: Colony Assay, Control

    Intracellular ROS levels in 4T1 cells following treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 48 h were analyzed by flow cytometry. Mean fluorescence intensity (FITC-A) was used to represent ROS levels. Data are expressed as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Isorhamnetin inhibits migration and promotes apoptosis via modulation of oxidative stress, mitochondrial dysfunction, and the TIGIT/CD155 axis in 4T1 breast cancer cells

    doi: 10.3389/fcell.2026.1829358

    Figure Lengend Snippet: Intracellular ROS levels in 4T1 cells following treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 48 h were analyzed by flow cytometry. Mean fluorescence intensity (FITC-A) was used to represent ROS levels. Data are expressed as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Article Snippet: The 4T1 mouse mammary carcinoma cell line and HC11 mouse thymic epithelial cell line were both purchased from KeyGEN BioTECH Co., Ltd., (Nanjing, China).

    Techniques: Flow Cytometry, Fluorescence, Control

    (a) Representative images of mitochondrial membrane potential in 4T1 cells following treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 48 h, assessed by JC-1 staining assay. (b) Quantitative analysis of mitochondrial membrane potential expressed as the red/green fluorescence intensity ratio. A decrease in the red/green ratio indicates loss of mitochondrial membrane potential. Data are presented as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Isorhamnetin inhibits migration and promotes apoptosis via modulation of oxidative stress, mitochondrial dysfunction, and the TIGIT/CD155 axis in 4T1 breast cancer cells

    doi: 10.3389/fcell.2026.1829358

    Figure Lengend Snippet: (a) Representative images of mitochondrial membrane potential in 4T1 cells following treatment with isorhamnetin (ISO) (0, 6, 12.5, and 25 μM) for 48 h, assessed by JC-1 staining assay. (b) Quantitative analysis of mitochondrial membrane potential expressed as the red/green fluorescence intensity ratio. A decrease in the red/green ratio indicates loss of mitochondrial membrane potential. Data are presented as mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.

    Article Snippet: The 4T1 mouse mammary carcinoma cell line and HC11 mouse thymic epithelial cell line were both purchased from KeyGEN BioTECH Co., Ltd., (Nanjing, China).

    Techniques: Membrane, Staining, Fluorescence, Control

    (a,b) mRNA analysis of TIGIT and CD155 in 4T1 cells treated with isorhamnetin (ISO) (0, 6, 12.5, and 25 µM) for 48 h. (c) Western blot image showing the expression of TIGIT and CD155 in 4T1 cells treated with ISO (0, 6, 12.5, and 25 µM) for 48 h. (d) Western blot analysis of TIGIT expression normalized to GAPDH in 4T1 cells treated with ISO (0, 6, 12.5, and 25 µM) for 48 h. (e) Western blot analysis of CD155 expression normalized to GAPDH in 4T1 cells treated with ISO (0, 6, 12.5, and 25 µM) for 48 h. Data are presented relative to the untreated control group and expressed as mean ± standard error (n = 3). Significance levels between the control group and experimental groups were defined as follows: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Isorhamnetin inhibits migration and promotes apoptosis via modulation of oxidative stress, mitochondrial dysfunction, and the TIGIT/CD155 axis in 4T1 breast cancer cells

    doi: 10.3389/fcell.2026.1829358

    Figure Lengend Snippet: (a,b) mRNA analysis of TIGIT and CD155 in 4T1 cells treated with isorhamnetin (ISO) (0, 6, 12.5, and 25 µM) for 48 h. (c) Western blot image showing the expression of TIGIT and CD155 in 4T1 cells treated with ISO (0, 6, 12.5, and 25 µM) for 48 h. (d) Western blot analysis of TIGIT expression normalized to GAPDH in 4T1 cells treated with ISO (0, 6, 12.5, and 25 µM) for 48 h. (e) Western blot analysis of CD155 expression normalized to GAPDH in 4T1 cells treated with ISO (0, 6, 12.5, and 25 µM) for 48 h. Data are presented relative to the untreated control group and expressed as mean ± standard error (n = 3). Significance levels between the control group and experimental groups were defined as follows: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The 4T1 mouse mammary carcinoma cell line and HC11 mouse thymic epithelial cell line were both purchased from KeyGEN BioTECH Co., Ltd., (Nanjing, China).

    Techniques: Western Blot, Expressing, Control